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<br>- [[Thyroglobulin]] is synthesized in the [[rough endoplasmic reticulum]] and follows the [[secretory pathway]] to enter the colloid in the lumen of the [[thyroid follicle]] by [[exocytosis]].
<br>- Meanwhile, a [[sodium-iodide symporter|sodium-iodide (Na/I) symporter]] pumps iodide (I<sup>-</sup>) [[active transport|actively]] into the cell, which previously has crossed the [[endothelium]] by largely unknown mechanisms.
<br>- This iodide enters the follicular lumen from the cytoplasm by the transporter [[pendrin]], in a purportedly [[passive transport|passive]] manner.<ref>[http://www.touchbriefings.com/pdf/2782/rousset.pdf How Iodide Reaches its Site of Utilisation in the Thyroid Gland – Involvement of Solute Carrier 26A4 (Pendrin) and Solute Carrier 5A8 (Apical Iodide Transporter)] {{Webarchive|url=https://web.archive.org/web/20111001092157/http://www.touchbriefings.com/pdf/2782/rousset.pdf |date=2011-10-01 }} - a report by Bernard A Rousset. Touch Brieflings 2007</ref>
<br>- In the colloid, iodide (I<sup>-</sup>) is [[Redox|oxidized]] to iodine (I<sup>0</sup>) by an enzyme called [[thyroid peroxidase]].
<br>- Iodine (I<sup>0</sup>) is very reactive and iodinates the thyroglobulin at [[tyrosyl]] residues in its protein chain (in total containing approximately 120 tyrosyl residues).
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